Nobelza single-use LoC cartridge has everything to carryout RT-PCR. Chip consists of six reaction wells, preloaded with lyophilized reagents. Any qPCR protocol developed for larger, more common instruments can be easily ported to Nobelza. Chip is compatible with purified DNA/RNA in aqueous solution, e.g., extracted from blood or other biological samples.
Thermal cycling can be programmed depending on the protocol, and is controlled by a microcontroller through a Proportional-Integrative-Derivative (PID) algorithm.
Radio-Frequency Identification (RFID) tag on the cartridge flipside allows for the storage of manufacturing information.
Size: 21.9 × 46.9 × 6.2 mm Weight: 4 grams Chip material: Polycarbonate Cartilage type: Silicon based RT-PCR Operational voltage: 5 V @ 100 mA Ramp rate: + 15 and -7 °C/s with control and thermal accuracy of ± 0.1 and ± 0.25 °C respectively. Cooling type: Peltier free passive airflow Wells: 6 Well volume: 5 µL Reagent type on chip: Lyophilised Storage: 4 °C Chip covering: poly(methyl methacrylate) sliding cap Contamination prevention: Wells preloaded with wax, which melts during the first heating step and floats on top